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The aim of this study was to evaluate the addition of an enzyme complex to the digestibility of annatto meal for Nile tilapia. A digestibility trial was conducted to evaluate the apparent digestibility coefficients of nutrients and digestible energy of annatto seed meal (FSU) and the effects of supplementing feed with an enzyme complex containing cellulase, protease and amylase on the digestibility of nutrients in juvenile nile tilapia (Oreochromis niloticus). The experimental rations were supplemented with a commercial enzyme complex at levels of 0.0, 0.033, 0.066 and 0.099%. The collection period lasted 15 days and apparent digestibility was determined using the indirect method, using chromium oxide (Cr2O3) as a digestibility indicator. A total of 45 male juvenile nile tilapia weighing approximately 100.00g were randomly distributed in three incubators adapted for digestibility testing.