Hames Glover, D. M. Glover

DNA Cloning

A Practical Approach Volume 2: Expression Systems
Herausgeber: Glover, David M.

• Broschiertes Buch

Produktbeschreibung

Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

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Produktdetails

• Verlag: OUP Oxford
• Second
• Seitenzahl: 276
• Erscheinungstermin: 4. März 2002
• Englisch
• Abmessung: 234mm x 156mm x 15mm
• Gewicht: 424g
• ISBN-13: 9780199634781
• ISBN-10: 0199634785
• Artikelnr.: 21218336

Inhaltsangabe

* 1.: Screening expression libraries with antibody and protein probes
* 2.: Expression of foreign proteins in E.coli using plasmid vectors
and purification of specific polyclonal antibodies
* 3.: Purification of over-produced proteins from E.coli cells
* 4.: Production of monoclonal antibodies against proteins expressed in
E.coli
* 5.: Expression of cloned genes in yeast
* 6.: Intraction trap cloning with yeast
* 7.: The baculovirus expression system