Hames Glover, D. M. Glover, David Hames

DNA Cloning

A Practical Approach Volume 1: Core Techniques

• Broschiertes Buch

Produktbeschreibung

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

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Produktdetails

• Verlag: OUP Oxford
• Second
• Seitenzahl: 288
• Erscheinungstermin: 1. März 2002
• Englisch
• Abmessung: 234mm x 156mm x 16mm
• Gewicht: 442g
• ISBN-13: 9780199634767
• ISBN-10: 0199634769
• Artikelnr.: 21428669

Inhaltsangabe

* 1.: Techniques for transformation of E.coli
* 2.: Lambda replacement vectors in the construction of genomic DNA
libraries
* 3.: Procedures for cDNA cloning
* 4.: Making probes
* 5.: Using cloned DNAs to analyse gene organization and expression
* 6.: Choices of single-stranded vectors and their application for in
vitro mutagenesis
* 7.: PCR and its applications
* 8.: DNA sequencing