Dieses Kursmanual beschreibt genetische Experimente an der Fruchtfliege Drosophila, die ohne aufwendige Ausrüstung in jedem Labor durchgeführt werden können. Es ist damit für Studentenpraktika ideal geeignet und ebenso für ein schnelles Screening auf Toxizität und Mutagenizität von neu entwickelten Substanzen.
Dieses Kursmanual beschreibt genetische Experimente an der Fruchtfliege Drosophila, die ohne aufwendige Ausrüstung in jedem Labor durchgeführt werden können. Es ist damit für Studentenpraktika ideal geeignet und ebenso für ein schnelles Screening auf Toxizität und Mutagenizität von neu entwickelten Substanzen.
1. General.- 1.1 Why Drosophila?.- 1.2 Basic Equipment.- 1.3 Genetic Terminology.- 1.4 The Biology of Drosophila melanoqaster.- 1.5 Methods for Culturing and Studying Drosophila melanoqaster.- 1.6 List of Genetic Symbols and Stocklist.- 1.7 General Books on Drosophila.- 2. Morphology of Drosophila Melanogaster.- 2.1 Eggs and Embryos.- 2.2 L3 Larvae.- 2.3 Pupae.- 2.4 Adult Flies.- 2.5 Some Mutants.- 3. Transmission Genetics.- 3.1 Dihybrid Cross with Independent Assortment.- 3.2 Sex-Linked Inheritance.- 3.3 Dihybrid Test Cross with Linked Genes.- 3.4 Sex Determination.- 3.5 Genetic Localization of Mutations Within the Genome.- 3.6 Mapping on a Chromosome.- 3.7 Segregation of Compound Chromosomes.- 3.8 Meiotic Mutants.- 4. Phenogenetics.- 4.1 Temperature Effect on Expression of Phenotype.- 4.2 Temperature Effect on a Homeotic Mutant.- 4.3 Mutants with Abnormal Eye Color.- 4.4 Chromatographic Analysis of Eye Color Mutants.- 4.5 Transplantation of Eye Imaginai Discs.- 4.6 Supplementation of an Eye Color Mutant.- 4.7 Genetic Complementation and Allelism.- 4.8 Transposable Elements.- 5. Mutation Genetics.- 5.1 Induction and Detection of Sex-Linked Recessive Lethals.- 5.2 Induction and Detection of Reciprocal Translocations.- 5.3 Mutagen-Induced Sex Chromosome Losses.- 5.4 Somatic Mutation and Mitotic Recombination.- 6. Population Genetics.- 6.1 Determination of Allele Frequencies in Populations in Hardy-Weinberg Equilibrium.- 6.2 Variations in Populations with Natural and Artificial Selection.- 6.3 Mating Behavior.- 6.4 Mating Preferences.- 7. Cytology and Cytogenetics.- 7.1 Microscopic Analysis of Mitotic and Polytene Chromosomes.- 7.2 Balancer Chromosomes.- 8. Molecular Biology of Drosophila: An Overview.- 9. Results and Answers.- 10. Subject Index.
1. General.- 1.1 Why Drosophila?.- 1.2 Basic Equipment.- 1.3 Genetic Terminology.- 1.4 The Biology of Drosophila melanoqaster.- 1.5 Methods for Culturing and Studying Drosophila melanoqaster.- 1.6 List of Genetic Symbols and Stocklist.- 1.7 General Books on Drosophila.- 2. Morphology of Drosophila Melanogaster.- 2.1 Eggs and Embryos.- 2.2 L3 Larvae.- 2.3 Pupae.- 2.4 Adult Flies.- 2.5 Some Mutants.- 3. Transmission Genetics.- 3.1 Dihybrid Cross with Independent Assortment.- 3.2 Sex-Linked Inheritance.- 3.3 Dihybrid Test Cross with Linked Genes.- 3.4 Sex Determination.- 3.5 Genetic Localization of Mutations Within the Genome.- 3.6 Mapping on a Chromosome.- 3.7 Segregation of Compound Chromosomes.- 3.8 Meiotic Mutants.- 4. Phenogenetics.- 4.1 Temperature Effect on Expression of Phenotype.- 4.2 Temperature Effect on a Homeotic Mutant.- 4.3 Mutants with Abnormal Eye Color.- 4.4 Chromatographic Analysis of Eye Color Mutants.- 4.5 Transplantation of Eye Imaginai Discs.- 4.6 Supplementation of an Eye Color Mutant.- 4.7 Genetic Complementation and Allelism.- 4.8 Transposable Elements.- 5. Mutation Genetics.- 5.1 Induction and Detection of Sex-Linked Recessive Lethals.- 5.2 Induction and Detection of Reciprocal Translocations.- 5.3 Mutagen-Induced Sex Chromosome Losses.- 5.4 Somatic Mutation and Mitotic Recombination.- 6. Population Genetics.- 6.1 Determination of Allele Frequencies in Populations in Hardy-Weinberg Equilibrium.- 6.2 Variations in Populations with Natural and Artificial Selection.- 6.3 Mating Behavior.- 6.4 Mating Preferences.- 7. Cytology and Cytogenetics.- 7.1 Microscopic Analysis of Mitotic and Polytene Chromosomes.- 7.2 Balancer Chromosomes.- 8. Molecular Biology of Drosophila: An Overview.- 9. Results and Answers.- 10. Subject Index.
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