Ajay Kumar

Dumbelled Oligodeoxynucleotides Inhibitors of RNaseH Activity

Reverse Transcriptase s RNase H Inhibitors

• Broschiertes Buch

Produktbeschreibung

Synthesis and characterization of dumbbell oligonucleotides containing loops of phosphodiester , two/ three trimethylene, C3 moieties in each loop and phosphorothioate linkages is described. Incubation of dumbbell oligonucleotides containing loops of phosphorothioate with S-1 did not result in significant cleavage. Binding of dumbbell oligonucleotides to MoMuL Virus reverse transcriptase was evaluated by PAGE band mobility shift assays. Dissociation constants evaluated using PAGE band mobility shift assays were of the order of 10-7. Investigation of inhibition of RNase H activity of reverse transcriptase showed that the dumbbell oligonucleotides containing loops of phosphorothioate are better inhibitor of the retroviral RNase H activity than the dumbbell oligonucleotides containing loops of phosphodiester , two/ three trimethylene, C3 moieties in each loop. Our studies demonstrated that the oligonucleotides containing loops of phosphorothioate displays an IC50 of 18- 25 nM in itsinhibition on the reverse transcriptase RNase H activity, a magnitude lower than that of first nucleotide reverse transcriptase of HIV-1, tenofovir, introduced by Gilead Science in the market.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Aufl.
• Seitenzahl: 92
• Englisch
• Abmessung: 220mm
• ISBN-13: 9783659110986
• ISBN-10: 3659110981
• Artikelnr.: 35689437

Autorenporträt

Dr. Ajay Kumar is a Professor in the Department of Chemistry, Galgotias University,UP. He earned his Ph.D from University of Delhi and had post doctoral training at University of Bordeaux,France and University of Rochester,USA. He has authored 50 research papers in National and International journals and 4 books.He is BOYSCAST Fellow,DST,India.