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The Real time monitoring plays an important role in biomedical engineering, particularly in ECG. The ECG signal is a quasi periodical, rhythmically repeating signal synchronized by the mechanical functioning of the heart. ECG is consisting of P, QRS, T and U wave of particular amplitude and duration's. Hence, in order to extract the characteristics features from the ECG by the use of Personal computer & to make it real time, we need a instrumentation system. One of them is based on LabVIEW. LABVIEW is general-purpose software for the virtual instrumentation, thorough LabVIEW data acquisition systems; we make the real time systems for monitoring of ECG with very high performance, Low cost of development, more reliable and flexible. This Book is introducing the designed aspects of the real time monitoring of the ECG signal on virtual cardiograph, consisting of four parts. Data acquisition of ECG signal. Filtering of data logger. Representations of Acquired ECG signal on virtual graphs. Preparation of data loggers.