The samples of maize grains collected from market showed A. niger and A. flavus dominant in all maize varieties and Aflatoxins were found above the legal limits of India and other countries. The selected maize varieties like Amber, Local and African Tall, mycoflora is responsible for deterioration of the seed health and production of harmful mycotoxins which found affecting seed germination and seedling growth. In order to protect above variety grains clove oil, cinnamon oil etc. has been used as pre-treatment of seeds before sowing. The research gives promising method for preservation in rural areas where they do not have any access to modern storage facilities. The present study also bridges gaps between the agricultural or plant sciences, human and animal health and environmental safety. Improved methods of application e.g., mechanical mixers for uniform and bulk coating of oil on grain, use of slow release dispensers/sachets which could be placed at different depths in storage structures, bins or bags, could be devised for ensuring and enhancing efficacy. Further study in this direction may be standardized many botanicals for its chemical and biological properties.