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The aim of this study was to evaluate in vitro motility and viability parameters when diluting the 5% post-thaw cryoprotectant dimethylformamide to concentrations of 2.5 and 1.25%, using two commercial diluents added to cryopreserved equine semen. After thawing, the samples were diluted in order to maintain the final concentrations (2.5 and 1.25%) of cryoprotectant, using two commercial diluents (FR4® and Botu-Crio®) at two times: initial (Ti) and final (Tf). Thirteen different ejaculate samples from five national breed stallions were used. Motility parameters were observed using computerized…mehr

Produktbeschreibung
The aim of this study was to evaluate in vitro motility and viability parameters when diluting the 5% post-thaw cryoprotectant dimethylformamide to concentrations of 2.5 and 1.25%, using two commercial diluents added to cryopreserved equine semen. After thawing, the samples were diluted in order to maintain the final concentrations (2.5 and 1.25%) of cryoprotectant, using two commercial diluents (FR4® and Botu-Crio®) at two times: initial (Ti) and final (Tf). Thirteen different ejaculate samples from five national breed stallions were used. Motility parameters were observed using computerized analysis and plasma membrane integrity using epifluorescence microscopy. There was an improvement in total and progressive sperm motility parameters at the final time (P<0.05) with Botu-Crio® diluent compared to FR4®. There was no difference, P>0.05, between the treatments in terms of plasma membrane integrity.
Autorenporträt
Sie hat einen Abschluss in Veterinärmedizin von der UFRuralRJ (2004) und einen Master in Agrarwissenschaften von der UnB (2007). Sie hat Erfahrung in der Veterinärmedizin. Sie hat Erfahrung in der Veterinärmedizin, im Bereich der Pathophysiologie der Tierreproduktion und der Biotechnologie, der Toxikologie, der Lehre, der Verteidigung der Tiergesundheit und der technischen Beratung beim Landwirtschaftsverband CNA.