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Infusion therapy is one of the major responsibilities of nurses. In dealing with intravenous infusion, nurses must have knowledge about what is ordered, why it is indicated, its intended impact on the patient, and any possible side effects that may occur. They also must understand the rationale for intravenous fluid administration and the type of intravenous solution ordered. Education and training are two components of nursing staff development that occur after an employee orientation. Thereby, this study was aimed to evaluate the effect of implementing intravenous infusion therapy protocol on nurses' knowledge and performance at Specialized Medical Hospital.