Nazanin Jafarpour, Mohammad Sina

Effect of miR-34a replacement

on inhibiting the growth and migration of colon cancer cells, HCT116

• Broschiertes Buch

Produktbeschreibung

Colorectal cancer as the most common cancer of the digestive tract is the fourth leading cause of death in the world. According to studies, miRNA-34a (miR-34a) expression declines in colon cancer. The aim of this study was to investigate the effect of miR-34a on growth and migration inhibition and apoptosis induction of colon cancer cells. In this experimental study, HCT116 human colorectal cancer cells were used in RPMI1640 medium. MiR-34a transmission into cancer cells was performed by the jetPEI transfection reagent. MTT assay to determine the survival of cancer cells and the qRT-PCR test to confirm the transfer of miR-34a to the cells and also an investigation of the expression of Caspase genes were used. Finally, the Wound healing test in order to investigate the migration status of transfected and control cells was performed. The expression changes of the intended genes in the transfected cells were compared with the control cells using the 2-¿¿Ct method.

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Produktdetails

• Verlag: Scholars' Press
• Seitenzahl: 84
• Erscheinungstermin: 26. November 2019
• Englisch
• Abmessung: 220mm x 150mm x 6mm
• Gewicht: 143g
• ISBN-13: 9786138918455
• ISBN-10: 6138918452
• Artikelnr.: 60210245

Autorenporträt

Nazanin Jafarpour-Bachelor¿s degree is Cellular & Molecular Biology -, Cellular & Molecular ,GPA out of 20 Higher Educational Institute of Rab-Rashid, Tabriz, Iran. MSc in Molecular genetics, GPA out of 20 Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran.