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All Biochemical and Physiological characters of Cotton plant of different level of Sulphur fertilizer Applied. The plant sample was collected 45 days after sowing and Soil sample was Analyzed before sowing and after harvesting. The experimental results and discussion pertaining to different biochemical and physiological parameters were investigated to understand the effect of different level of sulphur in cotton using RBD statistical design. Data were presented in tables or figure and also illustrated graphically wherever found necessary in this chapter under following main and sub heads.