The study was conducted to evaluate the efficacy of seaweed (Gracilaria sp.) as feed additives on specific growth rate, weight gain, survival and some biochemical parameters in rohu (Labeo rohita) for 45 days. The four isonitrogenous experimental diets with 30% crude protein were prepared by adding Gracilaria sp. at 0% (control diet), 10% (T1), 20% (T2) and 30% (T3) inclusion level. Total 400 number of L. rohita fries was randomly distributed into four treatments, each with quintuplicate. The daily ration i.e. 10% of total body mass was divided into two equal parts and was fed at 09.00 hrs and 17.00 hrs. Sampling was carried out every 15 days to monitor growth performance and to adjust rations. The water quality parameters viz. DO, alkalinity, pH and temperature was found in the permissible range during entire experimental period.