The present study "Influence of media and seed treatment chemicals on the enhancement of germination and seedling growth of papaya (Carica papaya L.) cv. Madhu Bindu" was carried out at Regional Horticultural Research Station, NAU, Navsari. The experiment was laid out in Completely Randomized Design with two factors repeated three times.The treatment comprised of four different media viz. red laterite soil + vermicompost (M1), red laterite soil + cocopeat (M2), red laterite soil + vermicompost + cocopeat (M3) and red laterite soil + vermicompost + sand (M4) and seven different seed treatment chemicals viz. GA3 100 mg/l (C1), GA3 200 mg/l (C2), NAA 100 mg/l (C3), NAA 200 mg/l (C4), thiourea 1000 mg/l (C5), thiourea 2000 mg/l (C6) and control (C7). The effect of different media and seed treatment chemicals on germination, growth, root and biochemical characters of papaya seedling were studied. Based on investigation use of media red laterite soil + vermicompost + cocopeat (1:1:1) and GA3 200 mg/l as seed treatment chemical can be used for better germination and growth of papaya seedling cv. Madhu Bindu.