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Articles in EGASP '05 are based on presentations made at the E-GASP 2005 gene prediction workshop, held in Hinxton, UK, on 6-7 May 2005. The workshop was community experiment to assess the state-of-the-art in genome annotation in the human genome, with the goal of assessing the accuracy of computational methods to predict protein coding genes in DNA and the overall assessment of the completeness of the current human genome annotations.