The First International School on "Electromagnetic Fields and Biomembranes" took place in Pleven, Bulgaria on 6-12 October 1986. It was designed as an advanced course through a collaboration of the Biological Faculty of Sofia University and the Council of the Bioelectrochemical Society. In an advanced course the lecturers are specialized in particular areas, and the students are usually specialists in related areas. We have captured the expertise of both groups of participants in this volume. The longer papers prepared by the lecturers are joined with the shorter papers based on the posters…mehr
The First International School on "Electromagnetic Fields and Biomembranes" took place in Pleven, Bulgaria on 6-12 October 1986. It was designed as an advanced course through a collaboration of the Biological Faculty of Sofia University and the Council of the Bioelectrochemical Society. In an advanced course the lecturers are specialized in particular areas, and the students are usually specialists in related areas. We have captured the expertise of both groups of participants in this volume. The longer papers prepared by the lecturers are joined with the shorter papers based on the posters presented by the "students" to provide a summary of the school as well as an indication of current research directions in the field. The course was designed to provide the latest information about biomembrane structure and function, covering the properties of both the lipid matrix and the recently characterized proteins that function as specialized channels and receptors. Real membranes and various models were covered, with an emphasis on understanding their mechanisms of interaction with various exogenous stimuli (e.g., electric, magnetic, light, etc.). Several practical applications of this information (e.g., electroporation, electro-fusion) were also presented with indications of the possibilities for new developments in biotechnology. The mixture of basic science with practical applications, together with the int~rmingling of lecturers and students from many different countries produced a stimulating atmosphere and effective teaching. We hope that this volume will transmit some of this atmosphere.
Content.- Membrane Lipids and their Model Systems.- Hydration Forces between Phospholipid Membranes and the Polyethylene Glycol Induced Membrane Approach.- Electrical Double Layers in Membrane Transport and Nerve Excitation.- An Alternative Electrostatic Model for Membrane Ion Channels.- Electroorientation Studies on Electrophysical Properties of Erythrocytes in Radio-Frequency Range of Alternating Electric Field.- Chagre Motion Inside Proteins.- Electroconformational Coupling and the Effects of Static and Dynamic Electric Field on Membrane Transport.- Cell Behaviour in Electric Field.- Topics of Bioelectrochemistry by Electromagnetic Field Effects.- Pulse Impedance Method and its Application in Medical Physics.- Locust Muscle Glutamate Receptors - Support for an Allosteric Model.- Basic Problems of Electromagnetic Biology.- ELF-Pulsating Magnetic Field /PEMF/-Induced Acoustic Effects in Vessel Walls - An Adequate Stimulation of Bioreceptors ?.- Magnetotherapy with Low-Frequency Electromagnetic Field.- Electromagnetic Fields - A New Ecological Factor.- Erythrocyte Shape is Induced by Free Electric and Chemical Energy.- The Electroluminescent Characteristics Correlate to the Age-Dependent Changes IN the Composition of Plasma Membrane.- Changes in Conformational State of Glycereldehyde-3-Phosphate Dehydrogenase Adsorbed on Phosphatidylinositol Liposomes.- Biological Activity of Macrophages Interacting with Small Liposomes and Micellar-Like Membrane Structures.- Concerning Some Variations of the Characteristics of Electrochemically Active Membrane after Pulse Magnetic and Electromagnetic Field Action.- Effects of Low Frequency Magnetic Fields on Chick Embryos and Embrional Chick Tibia.- The Influence of the Magnetic Field upon the Hatching Capacity of Poultry andUpon Plant Seeds.- The Geomagnetic Field as a Lowenergatic Factor with Important Biological Significance.- Electrorotation - Influence on Pulse Shape and Internal Membrane Systems on Rotational Spectra.- Electrorotation Measurements on Mouse Blastomers.- Effect of the Temperature on the Cell Contact by Dipolophoresis and Electrofusion.- The Effect of Surface Charged Modifiers on Halobacterium halobium Purple Membrane Phase Transitions.- Surface - Induced Orientation and Elasticity in Purple Membrane Thin Film Media.- A Study of the Fluorescence Spectra on Native Monomeric Bacteriorhodopsin in Purple Membrane.- HCO3-Stimulated ATPase Activity of Brain Mitochondria. The Role of Ca2+ and Mg2+ Ions Combination.- The Influence of Acetylcholine and Serotonin on the HCO3-ATPase Activity of Brain Mitochondria.- Effect of Chloramine-T on Single Sodium Channels in Neuroblastoma Cells.- A Recifying Potassium Channel in Human Neuroblastoma Cells.- Effect of Ions and ATP on the /3H/QNB Binding to Muscarinic Cholinenergic Receptors in Rat Brain Synaptic Membranes.- The Role of Some Cryoprotectants on the Survival of Rat Spermatozoa. A Study on the Membrane Fluidity.- Regulation of the Quantum Yield of Photosynthesis in Chlorela During Nutrient Limited Growth.- Surface Charge Density and Rotational Diffusion of Liposome Reconstituted Photosystem I Reaction Centres Studied by Spin-Label Technique.- Structural and Functional Characterization of the Effects of Detergent Treatment on Chloroplast Membranes.- The Heat Stability of the Photosynthetic Apparatus During the Process of High Temperature Acclimation.- Temperature Damage of the Oxygen-Evolving Complex in Thylakoid Membrane Particles.- Light Intensity Dependence of P700 Photooxidation in Heat-Stressed Pea Chloroplasts.- Effectsof Alpha-Tocopherol Derivatives on the Photosynthetic Activity of Thylakoid Membranes.- Metabolic Regulation Disturbance Mechanism of Nerve Cell Na-Pump in Radiation Pathology.- Luminometric Test for Human Erythrocyte ATP Monitoring after Gamma-Irradiation and Exogeneous ATP Administration.- Electrorotation of Lidocaine-Treated Numan Erythrocytes.- Spectral Characteristics of Chlorophyll A at Different States of Solution.- Electrophoretic Mobility of Myosin-Treated Limphocytes from Patients with Miocardial Infarction.- Lipid to Protein Ratio of Reconstituted Purple Membrane Fragments Determing the Electrokinetic Behaviour of Proteoliposomes.- Exogeneous ATP Administration Effects on Some Biophysical Properties of Human Erythrocytes During in Vitro Ageing.- Radioprotective Effects of ATP on In Vitro Gamma-Irradiated Human Red Blood Cells.- Electrophoretic Mobility Effects and Cell Size Distribution in Dependence on Nitroic Acid and Iron Content in Intensive Culture of Scenedesmus Acutus.- Influence of Constant Magnetic Field Upon Chemiluminesce in Wheat Roots.- Purple Membrane Fragments as a Model for Studying the Effects of Light Induced Electrophoretic Mobility Increase.- Author Index.
Content.- Membrane Lipids and their Model Systems.- Hydration Forces between Phospholipid Membranes and the Polyethylene Glycol Induced Membrane Approach.- Electrical Double Layers in Membrane Transport and Nerve Excitation.- An Alternative Electrostatic Model for Membrane Ion Channels.- Electroorientation Studies on Electrophysical Properties of Erythrocytes in Radio-Frequency Range of Alternating Electric Field.- Chagre Motion Inside Proteins.- Electroconformational Coupling and the Effects of Static and Dynamic Electric Field on Membrane Transport.- Cell Behaviour in Electric Field.- Topics of Bioelectrochemistry by Electromagnetic Field Effects.- Pulse Impedance Method and its Application in Medical Physics.- Locust Muscle Glutamate Receptors - Support for an Allosteric Model.- Basic Problems of Electromagnetic Biology.- ELF-Pulsating Magnetic Field /PEMF/-Induced Acoustic Effects in Vessel Walls - An Adequate Stimulation of Bioreceptors ?.- Magnetotherapy with Low-Frequency Electromagnetic Field.- Electromagnetic Fields - A New Ecological Factor.- Erythrocyte Shape is Induced by Free Electric and Chemical Energy.- The Electroluminescent Characteristics Correlate to the Age-Dependent Changes IN the Composition of Plasma Membrane.- Changes in Conformational State of Glycereldehyde-3-Phosphate Dehydrogenase Adsorbed on Phosphatidylinositol Liposomes.- Biological Activity of Macrophages Interacting with Small Liposomes and Micellar-Like Membrane Structures.- Concerning Some Variations of the Characteristics of Electrochemically Active Membrane after Pulse Magnetic and Electromagnetic Field Action.- Effects of Low Frequency Magnetic Fields on Chick Embryos and Embrional Chick Tibia.- The Influence of the Magnetic Field upon the Hatching Capacity of Poultry andUpon Plant Seeds.- The Geomagnetic Field as a Lowenergatic Factor with Important Biological Significance.- Electrorotation - Influence on Pulse Shape and Internal Membrane Systems on Rotational Spectra.- Electrorotation Measurements on Mouse Blastomers.- Effect of the Temperature on the Cell Contact by Dipolophoresis and Electrofusion.- The Effect of Surface Charged Modifiers on Halobacterium halobium Purple Membrane Phase Transitions.- Surface - Induced Orientation and Elasticity in Purple Membrane Thin Film Media.- A Study of the Fluorescence Spectra on Native Monomeric Bacteriorhodopsin in Purple Membrane.- HCO3-Stimulated ATPase Activity of Brain Mitochondria. The Role of Ca2+ and Mg2+ Ions Combination.- The Influence of Acetylcholine and Serotonin on the HCO3-ATPase Activity of Brain Mitochondria.- Effect of Chloramine-T on Single Sodium Channels in Neuroblastoma Cells.- A Recifying Potassium Channel in Human Neuroblastoma Cells.- Effect of Ions and ATP on the /3H/QNB Binding to Muscarinic Cholinenergic Receptors in Rat Brain Synaptic Membranes.- The Role of Some Cryoprotectants on the Survival of Rat Spermatozoa. A Study on the Membrane Fluidity.- Regulation of the Quantum Yield of Photosynthesis in Chlorela During Nutrient Limited Growth.- Surface Charge Density and Rotational Diffusion of Liposome Reconstituted Photosystem I Reaction Centres Studied by Spin-Label Technique.- Structural and Functional Characterization of the Effects of Detergent Treatment on Chloroplast Membranes.- The Heat Stability of the Photosynthetic Apparatus During the Process of High Temperature Acclimation.- Temperature Damage of the Oxygen-Evolving Complex in Thylakoid Membrane Particles.- Light Intensity Dependence of P700 Photooxidation in Heat-Stressed Pea Chloroplasts.- Effectsof Alpha-Tocopherol Derivatives on the Photosynthetic Activity of Thylakoid Membranes.- Metabolic Regulation Disturbance Mechanism of Nerve Cell Na-Pump in Radiation Pathology.- Luminometric Test for Human Erythrocyte ATP Monitoring after Gamma-Irradiation and Exogeneous ATP Administration.- Electrorotation of Lidocaine-Treated Numan Erythrocytes.- Spectral Characteristics of Chlorophyll A at Different States of Solution.- Electrophoretic Mobility of Myosin-Treated Limphocytes from Patients with Miocardial Infarction.- Lipid to Protein Ratio of Reconstituted Purple Membrane Fragments Determing the Electrokinetic Behaviour of Proteoliposomes.- Exogeneous ATP Administration Effects on Some Biophysical Properties of Human Erythrocytes During in Vitro Ageing.- Radioprotective Effects of ATP on In Vitro Gamma-Irradiated Human Red Blood Cells.- Electrophoretic Mobility Effects and Cell Size Distribution in Dependence on Nitroic Acid and Iron Content in Intensive Culture of Scenedesmus Acutus.- Influence of Constant Magnetic Field Upon Chemiluminesce in Wheat Roots.- Purple Membrane Fragments as a Model for Studying the Effects of Light Induced Electrophoretic Mobility Increase.- Author Index.
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