Reiner Westermeier

Electrophoresis in Practice

A Guide to Methods and Applications of DNA and Protein Separations
In collaboration w. Sonja Gronau, Phil Beckett et al.

• Gebundenes Buch

Produktbeschreibung

Gewohnt erfolgreiches Konzept, neues Gewand: Dieser klassische Leitfaden der Elektrophorese erhielt ein frisches Layout und wurde um interessante Informationen erweitert. So beschäftigt sich ein neuer Abschnitt mit der DIGE-Technologie, das Kapitel zur Proteomanalyse wurde umgestaltet und ausgebaut. Hinzu kommen zahlreiche aktuelle Literaturverweise.

Produktdetails

• Verlag: Wiley-VCH
• 4. Aufl.
• Seitenzahl: 406
• Englisch
• Abmessung: 246mm x 187mm x 26mm
• Gewicht: 940g
• ISBN-13: 9783527311811
• ISBN-10: 3527311815
• Artikelnr.: 05132468

Autorenporträt

R. Westermeier, Amersham Biosciences Europe GmbH, Freiburg, Germany

Born in 1951, Reiner Westermeier gained his diploma in engineering in 1976, and his doctorate in 1981 at the Technical University of Munich, Germany.
He remained at the TU engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours.
From 1984 to 1987 Dr. Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech since July 1997, and its Senior Scientist for Proteomics since January 2002.
Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.

Inhaltsangabe

PART I: FUNDAMENTALS

- Introduction

- Electrophoresis

- Isotachophoresis

- Isoelectric Focusing

- Blotting

- Interpretation of Electropherograms

- Proteome Analysis

- Instrumentation

PART II: EQUIPMENT AND METHODS

Equipment for Part II

Method 1: PAGE of Dyes

Method 2: Agarose and Immuno Electrophoresis

Method 3: Titration Curve Analysis

Method 4: Native PAGE in Amphoteric Buffers

Method 5: Agarose IEF

Method 6: PAGIEF in Rehydrated Gels

Method 7: Horizontal SDS-PAGE

Method 8: Vertical PAGE

Method 9: Semi-dry Blotting of Proteins

Method 10: IEF in Immobilized pH Gradients

Method 11: High-resolution 2D Electrophoresis

Method 12: PAGE of Double Stranded DNA

Method 13: Native PAGE of Single Stranded DNA

Method 14: Denaturing Gradient Gel Electrophoresis

Method 15: Denaturing PAGE of DNA

Appendix A: Trouble-shooting

References

Index