Emergence of Ndm-1 Among Carbapenem Resistant Klebsiella Pneumoniae
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Present study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in hospitals of Baghdad, Iraq. Fifty-five clinical isolates of K. K. pneumoniae resistant to carbapenem were investigated from burned wounds, sputum and blood samples. The susceptibility to different antibiotics was tested by the Vitek-2 system. All strains were multidrug resistant and they showed nine different antimicrobial resistant patterns (A- I). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29(52.73%) strains were MBL producing phenotypically, out of 55 carbapene...
Present study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in hospitals of Baghdad, Iraq. Fifty-five clinical isolates of K. K. pneumoniae resistant to carbapenem were investigated from burned wounds, sputum and blood samples. The susceptibility to different antibiotics was tested by the Vitek-2 system. All strains were multidrug resistant and they showed nine different antimicrobial resistant patterns (A- I). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29(52.73%) strains were MBL producing phenotypically, out of 55 carbapenem-resistant K. pneumoniae strains. The blaNDM-1 genes were detected by conventional PCR and the result showed 37(67.27%) strains positive for blaNDM-1 gene. Data obtained from blaNDM-1 gene sequencing was submitted to the GenBank under the accession numbers: KY524489, KY524488, KY497948, KY497947, KY501638 and KY501637.
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