Endocannabinoid Signaling

Methods and Protocols
Herausgegeben:Maccarrone, Mauro

• Gebundenes Buch

Produktbeschreibung

Thisvolume encompasses all major methodologies to interrogate endocannabinoidsystems (ECS) and endocannabinoids (eCBs) signaling. With increasing interesttowards the manifold activities of eCBs, this book discusses the chemical,biochemical, and molecular biological assays, and activity of distinct elementsof the ECS. These include membrane, nuclear receptors, biosynthetic andhydrolytic enzymes, and membrane transporters and oxidative enzymes. Written inthe highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists,molecular biologists, cell biologists, pharmacologists, and (electro)physiologists navigate themare magnum of endocannabinoid research.

Produktdetails

• Methods in Molecular Biology 1412
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3537-6
• 1st ed. 2016
• Seitenzahl: 300
• Erscheinungstermin: 1. Juni 2016
• Englisch
• Abmessung: 260mm x 183mm x 21mm
• Gewicht: 762g
• ISBN-13: 9781493935376
• ISBN-10: 1493935372
• Artikelnr.: 44072614

Inhaltsangabe

Need for Methods to InvestigateEndocannabinoid Signaling.- Extraction and Simultaneous Quantificationof Endocannabinoids and Endocannabinoid-Like Lipidsin Biological Tissues.- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS.- Analysis of Omega-3 Fatty AcidDerived N-acylethanolamines in BiologicalMatrices.- Assay of CB₁ Receptor Binding.- The Displacement Binding Assay Using HumanCannabinoid CB₂ Receptor-Transfected Cells.- Assay of TRPV1 Receptor Signaling.- A Functional Assay for GPR55: Envision Protocols.- The Cyclic AMP Assay Using Human CannabinoidCB₂ Receptor-Transfected Cells.- Assay of GTPGammaS Binding in Autoradiography.- Protocol to Study beta-Arrestin Recruitment by CB₁ and CB₂Cannabinoid Receptors.- Assay of NAT Activity.- Assay of NAPE-PLD Activity.- Assay of FAAH Activity.- Assay of NAAA Activity.- Assay of DAGL /beta Activity.- Assay of Monoacylglycerol Lipase Activity.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12.- Assay of Endocannabinoid Uptake.- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P_450.- Assessing Gene Expression of the Endocannabinoid System.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Traffickingin Cultured Cells.- Measuring ECS Interaction with Biomembranes.- Visualization of Endocannabinoids in the Cell.