Engineered Rice for enhanced Blast disease resistance - Jha, Sanjay;Jha, Anamika;Chattoo, Bharat Bhushan
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Transgenic rice with cleavable chimeric polyprotein was generated. The polyprotein precursor consists of a leader peptide and two different antimicrobial proteins (AMPs); Dm-AMP1 and Rs-AFP2. Plants transformed with polyprotein construct showed significantly improved disease resistance against Magnaporthe oryzae and Rhizoctonia solani. The chimeric polyprotein was cleaved and present in equimolar concentration in transgenic rice, and the individual AMPs were secreted into the extracellular space. The results of in vitro, in planta, and microscopic analyses suggest that chimeric polyprotein…mehr

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Produktbeschreibung
Transgenic rice with cleavable chimeric polyprotein was generated. The polyprotein precursor consists of a leader peptide and two different antimicrobial proteins (AMPs); Dm-AMP1 and Rs-AFP2. Plants transformed with polyprotein construct showed significantly improved disease resistance against Magnaporthe oryzae and Rhizoctonia solani. The chimeric polyprotein was cleaved and present in equimolar concentration in transgenic rice, and the individual AMPs were secreted into the extracellular space. The results of in vitro, in planta, and microscopic analyses suggest that chimeric polyprotein expression has the potential to provide broad-spectrum disease resistance in rice.
Autorenporträt
Dr. Sanjay Jha is a Plant Bio-technologist holding a Ph.D in Microbiology from M.S. University of Baroda, Gujarat, India. He is currently an Assistant Professor (Biotechnology)at ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Gujarat. The Author has published several papers in reputed peer reviewed journals.