Engineering of/with Lipases

Herausgegeben:Malcata, F. X.

• Broschiertes Buch

Produktbeschreibung

This book attempts to (i) systematically review the state of the art pertaining to the knowledge on the various levels of structure and catalytic function of Iipases, and (ii) discuss in a simultaneously pragmatic and integrated manner the design and modification of Iipases and the technological aspects of the utilization of Iipases for desired practical purposes. In preliminary assessments of whether the current knowledge of Jipases (and users thereof) would deserve (and benefit from) comprehensive academic and scholastic efforts eventually leading to the production of a formal book, four major issues were addressed: -Need. Although several applications of Iipases have been developped throughout the latest two decades, innovative uses of these enzymes for a wide variety of organic syntheses and modification of existing fats and oils have increased exponentially during the latest five years. Reasons which may partly account for this trend are the increasing availability of Jipases from (genetically engineered) microbial sources coupled with their special capacity to act as catalysts at hydrophilic / hydrophobic interfaces. As a result of the structural characterisation effected during the same period, applications of Jipases can now be developed in a much more rational fashion; however, this purpose will not be fully achieved unless careful attempts to comprehensively clarify and integrate such advances are developed. -Importance. Once viewed as solutions in search of problems, Iipases have conquered a special position in the field of biotechnology since the advent of enzyme catalyzed reactions in nonaqueous (or micro aqueous) media.

Produktdetails

• Nato Science Series E: 317
• Verlag: Springer / Springer Netherlands / Springer, Berlin
• Artikelnr. des Verlages: 978-94-010-7248-9
• Softcover reprint of the original 1st ed. 1996
• Seitenzahl: 640
• Erscheinungstermin: 26. September 2011
• Englisch
• Abmessung: 240mm x 160mm x 35mm
• Gewicht: 949g
• ISBN-13: 9789401072489
• ISBN-10: 9401072485
• Artikelnr.: 36119028

Inhaltsangabe

Overview.- Engineering of/with lipases: scope and strategies.- Selectivities: fundamentals and applications.- Selectivity of lipases: types and determination.- Selectivity of lipases: developmental physiology aspects - the role of lipase selectivity in the digestion of milk fat.- Lipase-catalyzed reactions for the fractionation of fatty acids.- Lipase specificities towards fatty acids: erucic acid and ?-linolenic acid.- Synthesis of chiral drugs using lipases.- Structure.- Structural characterization of a prokaryotic lipase.- Candida rugosa lipase isozymes: cloning, sequencing, analysis of the substrate binding pocket.- Structure/function relationships.- Lipases and esterases: some evolutionary and protein engineering aspects.- The kinetics, specificities and structural features of lipases.- Action of lipases.- Molecular engineering.- Strategies and design of mutations in lipases.- Protein engineering of microbial lipases with industrial interest.- Protein engineering of a fungal lipase: towards expression of a recombinant Candida rugosa lipase.- Interfacial activation-based molecular bioimprinting: towards a more rational use of lipolytic enzymes in nonaqueous media.- Modulation of activity/stability properties of lipase from Pseudomonas fluorescens by multipoint covalent immobilization on glyoxyl-supports.- Utilization of unfolding/refolding strategies for reactivation of immobilized derivatives of lipases after inactivation by organic solvents.- Kinetics and thermodynamics.- Chromatographic and spectrometric methods for the determination of the chemical composition of lipase-modified fats.- Kinetics of lipase catalyzed reactions in non conventional media.- Kinetics and stability of cutinase immobilized in reversed micelles and zeolites.- Thermodynamic principlesof enzymatic esterification.- Role of water.- Lipases in biphasic solid/liquid media.- Engineering of/with lipases: importance of water in new biocatalytic processes - the solid/gas catalysis example.- Applications.- Plant lipases in lipid biotransformations.- Lipase-catalyzed reactions under microaqueous conditions.- Wax synthesis by esterification and alcoholysis.- Glyceride synthesis from free fatty acids and glycerol.- Reactors.- Reactors with immobilized lipase: mathematical modelling.- Lipase membrane reactor for continuous hydrolysis of tallow.- Membrane bioreactor design to force equilibrium towards a favourable product yield.- Reversed micellar membrane bioreactor.- Bioreactors for continuous enzymatic esterification with insitu water activity control - I.- Bioreactors for continuous enzymatic esterification with insitu water activity control - II.- Scale-up of two-phase membrane reactors: use of free and immobilised lipase.- Separation/reaction.- Separation and purification of lipases by liquid-liquid extraction processes.- Lipases in water-in-oil microemulsions, organogels and Winsor II systems: aspects of reactivity and separation science.- Integration of reaction and separation with lipases: biocatalytic distillation.- Reaction/separation process in supercritical CO2 using lipases.