The last few decades have seen a flurry of activity in the synthesis and development of the Amylolytic enzymes from bacteria and fungi their production and characterization. Various strategies can be envisaged to synthesize by exploiting the reactivity of this organisms with their derivatives by various researchers. The aim of this book is to provide a brief knowledge about starch degrading bacteria and their application in commercially important amylolytic enzyme has wide industrial application due to its property of remaining active in broad range of pH as well as temperature. Thus amylase produced from a natural soil isolate, culture B5 is a commercially important amylolytic enzyme. We hope the readers can enjoy this text and find it useful in their respective studies.