Enhancers and Promoters

Methods and Protocols
Herausgeber: Giaimo, Benedetto Daniele; Borggrefe, Tilman

• Broschiertes Buch

Produktbeschreibung

This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting.Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments. Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

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Produktdetails

• Methods in Molecular Biology Nr.2351
• Verlag: Springer US / Springer US, New York, N.Y.
• 1st ed. 2021
• Seitenzahl: 392
• Erscheinungstermin: 14. August 2022
• Englisch
• Abmessung: 254mm x 178mm x 22mm
• Gewicht: 735g
• ISBN-13: 9781071615997
• ISBN-10: 1071615998
• Artikelnr.: 64361927

Inhaltsangabe

A New Toolbox to Analyze Enhancer
Promoter Functions.
 Global Run
on Sequencing (GRO
Seq).
 Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET
Seq).
 Low Quantity single strand CAGE (LQ
ssCAGE) Maps Regulatory Enhancers and Promoters.
 Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase
Seq.
 Measuring Chromatin Accessibility: ATAC
Seq.
 High
resolution ChIP
MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.
 Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.
 Low Input Targeted Chromatin Capture (low
T2C).
 Proximity Ligation
assisted ChIP
Seq (PLAC
Seq).
 Analysis of Enhancer
promoter Interactions using CAGE and RADICL
Seq technologies.
 Using Open Chromatin Enrichment and Network Hi
C (OCEAN
C) to identify open chromatin interactions.
 Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi
C (PCHi
C).
 Native Chromatin Proteomics (N
ChroP) to Characterize Histone Post
Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.
 Using ChIP
SICAP to Identify Proteins That Co
Localize in Chromatin.
 Genome
wide profiling of protein
DNA Interactions with Chromatin Endogenous Cleavage And High
Throughput Sequencing (ChEC
Seq).
 Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.
 Deletion of Regulatory Elements with All
in
One CRISPR
Cas9 Vectors.
 Simultaneous Tagmentation
based detection of ChIP/ATAC Signal with Bisulfite Sequencing.
 Low Input Whole
Genome Bisulfite Sequencing.