One corn (Zea maize L.) cultivar was used in this study (Giza 643), for its competence during the course of tissue culture, to improve its tolerance to salt stress via genetic transformation using glycerol-3-phosphate dehydrogenase (GPD1) gene isolated from Egyptian isolates of yeast (Saccharomyces cerevisiae) grown under salt and osmotic stress at concentrations 2M NaCl and 2M sorbitol, respectively. using simple and economic technique called Fluorescence differential display (FDD).