Enzyme Engineering and Evolution: General Methods

Herausgegeben:Tawfik, Dan S.

• Gebundenes Buch

Produktbeschreibung

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Produktdetails

• Methods in Enzymology Volume 643
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0015-2
• Englisch
• Abmessung: 20mm x 152mm x 229mm
• Gewicht: 700g
• ISBN-13: 9780128211496
• Artikelnr.: 67579065

Autorenporträt

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Inhaltsangabe

1. In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis



Javier Viña-Gonzalez and Miguel Alcalde



2. A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering



Haoran Yu and Paul A. Dalby



3. Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterization



Michal Vasina, Pavel Vanacek, Jiri Damborsky and Zbynek Prokop



4. Emulsion-based directed evolution of enzymes and proteins in yeast



Elizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar and Andrew D. Ellington



5. Remodeling enzyme active sites by stepwise loop insertion



Md Anarul Hoque, Yong Zhang, Li Zhi, Li Cui and Yan Feng



6. Consensus Finder web tool to predict stabilizing substitutions in proteins



Bryan J. Jones, Chi Nok Enoch Kan, Christine Luo and Romas J. Kazlauskas



7. The use of consensus sequence information to engineer stability and activity in proteins



Matt Sternke, Katherine W. Tripp and Doug Barrick



8. Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme



Jung Min Choi and Hak-Sung Kim



9. Biological fitness landscapes by deep mutational scanning



Jacob D. Mehlhoff and Marc Ostermeier



10. Focused rational iterative site-specific mutagenesis (FRISM)



Danyang Li, Qi Wu and Manfred T. Reetz



11. Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins



Charlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson and Nobuhiko Tokuriki



12. Machine learning-assisted enzyme engineering



Niklas E. Siedhoff, Ulrich Schwaneberg and Mehdi D. Davari



13. Ultrahigh throughput screening for enzyme function in droplets



Stefanie Neun, Paul J. Zurek, Tomasz S. Kaminski and Florian Hollfelder