Enzyme Engineering and Evolution: Specific Enzyme Applications

Herausgegeben:Tawfik, Dan S.

• Gebundenes Buch

Produktbeschreibung

Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.

Produktdetails

• Methods in Enzymology Volume 644
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0017-6
• Englisch
• Abmessung: 18mm x 152mm x 229mm
• Gewicht: 610g
• ISBN-13: 9780128244319
• Artikelnr.: 67578311

Autorenporträt

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Inhaltsangabe

1. Engineering lytic polysaccharide monooxygenases (LPMOs)



Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink



2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases



Daniela M. Carminati, Eric J. Moore and Rudi Fasan



3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries



Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer



4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution



Duncan S. Macdonald and Donald Hilvert



5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling



Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier



6. Engineering aldolases for asymmetric synthesis



Mikael Widersten



7. Ultra-high throughput screening for novel protease specificities



Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke



8. Directed evolution of transcriptional switches using dual-selector systems



Yuki Kimura and Daisuke Umeno



9. Methods for competitive enrichment and evaluation of superior DNA ligases



Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley



10. Directed evolution of custom polymerases using droplet microfluidics



Derek Vallejo, Ali Nikoomanzar and John C. Chaput



11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution



Pu Xue, Tong Si and Huimin Zhao



12. Split enzymes: Design principles and strategy



Shion A. Lim and James A. Wells