The study aimed to evaluate the chemical profile and larvicidal activity of essential oil (EO) and oil-in-water (O/W) nanoemulsions from Eucalyptus globulus Labil leaves and Ocimum africanum Lour. A significant total phenolic content was found to be correlated with an efficient antioxidant activity. Essential oils did not show high toxicity in toxicity tests. The larvicidal action of the EO of E. globulus and O. africanum was observed where LC50 of 26.58 mg L-1 and LC50 of 35.89 mg L-1, respectively, and their nanoemulsions with LC50 of 12.01 mg L-1 and 14.75 mg L-1 were found. Through the results obtained, it was proved larvicidal and improved action of nanoemulsions in the tests performed. Finally, this study points out the efficient larvicidal activity against Aedes aegypti larvae, and its application in arbovirus vector control areas is encouraged.