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Biosafety during lab work is an important concern in developing countries. Some critical issues concerning biosafety are ;lack of personal training, either exceeding workload or working too fast, deciding not to follow safe practices, and skepticism about biohazards. This study aimed to determine the level of biosafety perception and practices of laboratory technicians during routine work in clinical laboratories at Khartoum state primary health care centers, Sudan. Also there is a political attention that appeared healthy to reactivate the role of strategic primary health care centers which have laboratories in the state of Khartoum because of their leader role in education, treatment, promotion and rehabilitation.