Sickle cell disease is one of the most causes of morbidity and mortality among children.Sickling test is still the mainstay tool for diagnosis of Hb S ,the highlight of the book lies on evaluation of sickling test in diagnosis of sickle cell disease by knowing of sensitivity and specificity of sikling test in compare with hemoglobin electrophoresis as standard method. Determination of sensitivity and specificity of scikling test will helps to choose the appropriate method for diagnosis of sickle cell disease. Extremely useful for medical laboratory students and researchers working in hematology.