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The aim of this book was to establish the uptake, output and outcome of prevention of Mother to Child Transmission of Human immuno-deficiency virus. A total of 146 antenatal and intranatal mothers were targeted for the study. A descriptive cross sectional study was conducted. Sampling was done using systematic sampling. The method that was used for data collection was exist interview and also data abstraction form was used to collect data from patient's records. The data was collected and entered into computer packages for analysis and presentation. The SPSS statistical program was used for analysis.Student s t-test was used to compare continuous variables and Chi-square test for categorical variables.The results of this study have shown that uptake of PMTCT services at Kakamega Provincial hospital was good and most clients were satisfied with the PMTCT services offered. However, it was not be easy to tell from this study whether there have been a reduction in mother-to-child transmission of HIV on the program or not. This was because there was no baseline data prior to the initiation of the program and also there was more than 60% dropout rate during follow-up of children.