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Brazil has become the largest consumer of pesticides in the world, accounting for 86% of these products in the Latin American market. Tobacco is one of the crops that uses pesticides on a large scale to increase its quality and productivity. Exposure to pesticides without the necessary care and over the long term can cause acute or chronic poisoning, triggering a series of illnesses. In this respect, the identification of biomarkers that may indicate a potential physiological impairment of the organism and preventive measures are extremely important for the health of rural workers exposed to these substances.