Extracellular Vesicles

Herausgegeben:Spada, Sheila; Galluzzi, Lorenzo

• Gebundenes Buch

Produktbeschreibung

Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.

Produktdetails

• Methods in Enzymology Volume 645
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0016-4
• Englisch
• Abmessung: 18mm x 152mm x 229mm
• Gewicht: 610g
• ISBN-13: 9780128206621
• Artikelnr.: 67578558

Autorenporträt

Sheila Spada is at Weill Cornell Medical College, NY, USA

Inhaltsangabe

1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cells



Daniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu



2. Fluorescent labeling of extracellular vesicles



Mehdi Dehghani and Thomas R. Gaborski



3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells



Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai



4. Analysis of individual extracellular vesicles by imaging flow cytometry



Tobias Tertel, André Görgens and Bernd Giebel



5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy



Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci



6. Methods to purify DNA from extracellular vesicles: Focus on exosomes



Sheila Spada



7. Quantification of mtDNA in extracellular vesicles



Beata Soltes and Balint Nagy



8. Deciphering the total RNA content of extracellular vesicles



Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes



9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids



Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais



10. Isolation and characterization of human amniotic fluid-derived exosomes



Samantha Sheller-Miller and Ramkumar Menon



11. Human urine exosomes: Another important member of the liquid biopsy family



Antonio De Maio



12. Three-dimensional cell models for EV production, isolation, and characterization



Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko



13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles



Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya



14. Live tracking of extracellular vesicles in larval zebrafish



Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz



15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos



Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari