This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of standard microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of standard microbiology protocols that have to be modified to…mehr
This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of standard microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of standard microbiology protocols that have to be modified to be suitable for handling extremophiles. * Guides newcomers in the field of extremophile microbiology in the techniques used * Provides an overview of all areas of extremophile research * Many protocols could be adapted for use with a wide range of organisms
THERMOPHILES In-situ activity studies in thermal environments The isolation of thermophiles from deep-sea hydrothermal environments Growth of hyperthermophilic microorganisms for physiological and nutritional studies Toward the large scale cultivation of hyperthermophiles at high temperature and high-pressure The chemotaxonomic analysis of lipids from extremophilic bacteria Membranes of thermophiles and other extremophiles Characterization and quantification of compatible solutes in (hyper)thermophilic microorganisms Functional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricus Heat shock proteins in hyperthermophiles Deep-sea Thermococcales and their genetic elements: plasmids and viruses Genetic systems for Thermus Gene transfer systems for obligately anaerobic thermophilic bacteria Hyperthermophilic virus-host systems: detection and isolation Preservation of thermophilic microorganisms PSYCHROPHILES Handling psychrophilic microorganisms Proteins from psychrophiles ALKALIPHILES Cultivation of aerobic alkaliphiles Isolation, cultivation, and characterization of alkalithermophiles ACIDOPHILES The isolation and study of acidophilic microorganisms HALOPHILES Characterization of natural communities of halophilic microorganisms Cultivation of haloarchaea Halophile extraction from ancient crystals The assessment of the viability of halophilic microorganisms in natural communities Characterization of lipids of halophilic Archaea Characterization of organic compatible solutes of halotolerant and halophilic organisms Genetic systems for halophilic archaea The isolation ?of viruses of halophilic microorganisms Detection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophiles The storage of halophilic bacteria BAROPHILES Handling piezophilic microorganisms RADIATION-RESISTANT MICROORGANISMS Measuring survival in microbial populations following exposure to ionizing radiation STRICT ANAEROBES Strictly anaerobic microorganisms APPLICATIONS OF EXTREMOPHILES Industrial screening of extemophiles for valuable biomolecules
THERMOPHILES In-situ activity studies in thermal environments The isolation of thermophiles from deep-sea hydrothermal environments Growth of hyperthermophilic microorganisms for physiological and nutritional studies Toward the large scale cultivation of hyperthermophiles at high temperature and high-pressure The chemotaxonomic analysis of lipids from extremophilic bacteria Membranes of thermophiles and other extremophiles Characterization and quantification of compatible solutes in (hyper)thermophilic microorganisms Functional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricus Heat shock proteins in hyperthermophiles Deep-sea Thermococcales and their genetic elements: plasmids and viruses Genetic systems for Thermus Gene transfer systems for obligately anaerobic thermophilic bacteria Hyperthermophilic virus-host systems: detection and isolation Preservation of thermophilic microorganisms PSYCHROPHILES Handling psychrophilic microorganisms Proteins from psychrophiles ALKALIPHILES Cultivation of aerobic alkaliphiles Isolation, cultivation, and characterization of alkalithermophiles ACIDOPHILES The isolation and study of acidophilic microorganisms HALOPHILES Characterization of natural communities of halophilic microorganisms Cultivation of haloarchaea Halophile extraction from ancient crystals The assessment of the viability of halophilic microorganisms in natural communities Characterization of lipids of halophilic Archaea Characterization of organic compatible solutes of halotolerant and halophilic organisms Genetic systems for halophilic archaea The isolation ?of viruses of halophilic microorganisms Detection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophiles The storage of halophilic bacteria BAROPHILES Handling piezophilic microorganisms RADIATION-RESISTANT MICROORGANISMS Measuring survival in microbial populations following exposure to ionizing radiation STRICT ANAEROBES Strictly anaerobic microorganisms APPLICATIONS OF EXTREMOPHILES Industrial screening of extemophiles for valuable biomolecules
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