Fenugreek is one of the well known spices in human food. Its seeds and green leaves are used in food as well as in medicinal application. There are different varieties of fenugreek plan to cultivate based on soil and climatic conditions. The seeds of kasturi methi contain higher amount of cardiac glycoside and steroid whereas moderate amount of flavonoids and terpenoids. Flavonoids and steroids were present in all genotypes whereas tannin was only present in kasturi methi. The genotypes JFG.250 and JFG.266 contain higher amount of flavonoids, cardiac glycoside and terpenoids. Kasturi methi registered with higher RWC, phenol, crude protein and Total antioxidant activity. Seeds of PEB have maximum ash and free amino acids. From bio-molecular analysis of leaf it can be concluded from present experiment that JFG.256 recorded higher amount of flavonoids, cardiac glycosides, steroids and phenol content. Leaves of PEB possess higher content of protein, chlorophyll and carotenoids. Kasturi methi contain higher amount of total antioxidant activity. Thus these genotypes may be further use as breeding purpose for it's higher nutraceutical values.