The experiment was conducted at Experimental Research Farm,Department of Agricultural Entomology, Vasantrao Naik Marathwada KrishiVidyapeeth, Parbhani, during the kharif season of the year 2019-20 to studypopulation dynamics and bio-efficacy of biopesticides against major pests of chilli.The experiment was laid out in a randomized block design with 7 treatmentsreplicated three times. The treatments comprised of T1 (Neem oil @ 5 ml/L), T2(NSKE 5% @ 5 g/L), T3 (Dashparni ark @ 6 ml/L), T4 (Beauveria bassiana @ 4g/L),T5 (Verticilium lecanii @ 4g/L) and T6 (Metarrhizium anisopliae @ 4 g/L) and alongwith untreated check during kharif season of 2019-2020. The overall results indicated that the treatment with T1 (Neem oil @ 5 ml/L), T2 (NSKE 5% @ 5 g/L) and T5 (Verticilium lecanii @ 4g/L) was found superior in controlling chilli thrips, whitefly & mites whereas the treatment with T1 (Neem oil @ 5 ml/L), T2 (NSKE 5% @ 5 g/L) and T4 (Beauveria bassiana @ 4g/L) was found superior in controlling chilli fruit borer. In case of natural enemies the treatments T1 (Neem oil @ 5ml/L) andT2 (NSKE @ 5 ml/L) and T4 (Beauveria bassiana @ 4g/L) was proved to be more safer.