Flow Cytometry for Biotechnology - Sklar, Larry A. (ed.)
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Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collectedthrough a microscope objective, spectrally filtered, and detected with photomultiplier tubes.

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Produktbeschreibung
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collectedthrough a microscope objective, spectrally filtered, and detected with photomultiplier tubes.
Autorenporträt
Larry Sklar is Regents Professor of Pathology at the University of New Mexico. He is also Director of Basic Research at the University of New Mexico Cancer Research and Treatment Center.