This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and…mehr
This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
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Inhaltsangabe
Flower development in Arabidopsis.- Flower development in the Solanaceae.- The ABC of flower development in monocots: the model of rice spikelet.- Model species to investigate the origin of flowers.- Hormones and flower development in Arabidopsis.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice.- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize.- Genetic and phenotypic analyses of carpel development in Arabidopsis.- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis.- Floral induction systems for the study of Arabidopsis flower development.- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types.- Protoplast Isolation for Plant Single-Cell RNA-seq.- Plant Nuclei Isolation for Single-nucleus RNA Sequencing.- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis.- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution.- The GUS reporter system in flower development studies.- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species.- Gene expression analysis by quantitative real-time PCR for floral tissues.- Misexpression approaches for the manipulation of flower development.- Genomic approaches for the study of flower development in floriculture crops.- Multi-omics methods applied to flower development.- Peptidomics methods applied to the study of flower development.- Quantifying gene expression domains in plant shoot apical meristems.- A NanoLuc-based transactivation assay in plants.- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.
Flower development in Arabidopsis.- Flower development in the Solanaceae.- The ABC of flower development in monocots: the model of rice spikelet.- Model species to investigate the origin of flowers.- Hormones and flower development in Arabidopsis.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice.- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize.- Genetic and phenotypic analyses of carpel development in Arabidopsis.- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis.- Floral induction systems for the study of Arabidopsis flower development.- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types.- Protoplast Isolation for Plant Single-Cell RNA-seq.- Plant Nuclei Isolation for Single-nucleus RNA Sequencing.- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis.- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution.- The GUS reporter system in flower development studies.- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species.- Gene expression analysis by quantitative real-time PCR for floral tissues.- Misexpression approaches for the manipulation of flower development.- Genomic approaches for the study of flower development in floriculture crops.- Multi-omics methods applied to flower development.- Peptidomics methods applied to the study of flower development.- Quantifying gene expression domains in plant shoot apical meristems.- A NanoLuc-based transactivation assay in plants.- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.
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