Fluorescence In Situ Hybridization (FISH)
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This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear…mehr

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Produktbeschreibung
This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection.
This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working inthe fields of human genetics, microbiology, animal and plant sciences.

Autorenporträt
>50 book chapters, >550 peer-reviewed papers, and ~800 abstracts. His particular expertiseis molecular cytogenetics including chromosomal heteromorphisms, small supernumerary marker chromosomes (sSMC) and uniparental disomy, fields for which he has collected all available literature and made it freely available on the Internet. Further, Dr. Liehr has multiple longstanding international collaborations with many scientists around the world including researchers in e.g. Armenia, Croatia, India, Morocco, Russia, Serbia, Thailand, and Brazil.