"Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific…mehr
"Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.
Fluorescence spectroscopy has found wide spread application in many areas of science and medicine, including physics, chemistry, biology, biochemistry, clinical medicine, cytology, immunology, hydrogeology, and environmental sciences. The book presents recent applications of and brand new developments in fluorescence spectroscopy written by experts in the field. It is an invaluable source of information for experienced spectroscopists as well as researchers from other disciplines applying fluorescence techniques and for novices entering this field.
Inhaltsangabe
1. New Methods in Fluorescence Spectroscopy.- 1. Fluorescence Spectroscopy: Where We Are and Where We're Going.- 2. Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy.- 3. Fast Optical Imaging Techniques.- 4. Kinetic Studies on Fluorescence Probes Using Synchrotron Radiation.- 5. Dynamics and Geometry in Dimeric Flavoproteins from Fluorescence Relaxation Spectroscopy.- 6. Fluorescence Spectroscopy of Light Scattering Materials.- 7. Optical Sensors Based on Fluorescence Quenching.- 2. New Applications of Fluorometry.- 8. Fluorescence in Forest Decline Studies.- 9. Fluorescence Microscopy Studies of Structure Formation in Surfactant Monolayers.- 10. Fluorescence Lifetime Imaging and Application to Ca2+ Imaging.- 11. Ether Phospholipids in Membranes: Applications of Phase and Steady-State Fluorometry.- 12. Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models.- 13. Optical Detection of Intracellular Ion Concentrations.- 3. Fluorimetric Analysis.- 14. Analytical Applications of Very Near-IR Fluorimetry.- 15. Fluorescence Detection in Flow Injection Analysis.- 16. Fluorescence Spectroscopy in Environmental and Hydrological Sciences.- 4. Fluorescence Immunoassay.- 17. Fluorescence Polarisation Immunoassay.- 18. Progress in Delayed Fluorescence Immunoassay.- 19. Chemiluminescence Detection in Immunochemical Techniques. Applications to Environmental Monitoring.- 5. Fluorescence in Biomedical Sciences.- 20. Fluorescence Transients in Neurobiology: Applications of Voltage Sensitive and Ion Indicator Dyes.- 21. Optical Monitoring of Postsynaptic Potential in the Early Embryonic Avian Brain Stem Using a Voltage-Sensitive Dye.
1. New Methods in Fluorescence Spectroscopy.- 1. Fluorescence Spectroscopy: Where We Are and Where We're Going.- 2. Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy.- 3. Fast Optical Imaging Techniques.- 4. Kinetic Studies on Fluorescence Probes Using Synchrotron Radiation.- 5. Dynamics and Geometry in Dimeric Flavoproteins from Fluorescence Relaxation Spectroscopy.- 6. Fluorescence Spectroscopy of Light Scattering Materials.- 7. Optical Sensors Based on Fluorescence Quenching.- 2. New Applications of Fluorometry.- 8. Fluorescence in Forest Decline Studies.- 9. Fluorescence Microscopy Studies of Structure Formation in Surfactant Monolayers.- 10. Fluorescence Lifetime Imaging and Application to Ca2+ Imaging.- 11. Ether Phospholipids in Membranes: Applications of Phase and Steady-State Fluorometry.- 12. Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models.- 13. Optical Detection of Intracellular Ion Concentrations.- 3. Fluorimetric Analysis.- 14. Analytical Applications of Very Near-IR Fluorimetry.- 15. Fluorescence Detection in Flow Injection Analysis.- 16. Fluorescence Spectroscopy in Environmental and Hydrological Sciences.- 4. Fluorescence Immunoassay.- 17. Fluorescence Polarisation Immunoassay.- 18. Progress in Delayed Fluorescence Immunoassay.- 19. Chemiluminescence Detection in Immunochemical Techniques. Applications to Environmental Monitoring.- 5. Fluorescence in Biomedical Sciences.- 20. Fluorescence Transients in Neurobiology: Applications of Voltage Sensitive and Ion Indicator Dyes.- 21. Optical Monitoring of Postsynaptic Potential in the Early Embryonic Avian Brain Stem Using a Voltage-Sensitive Dye.
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