This updated volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and…mehr
This updated volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Foodborne Bacterial Pathogens: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Direct or DNA extraction-free amplification and quantification of foodborne pathogens.- The use of multiplex real time PCR for the simultaneous detection of foodborne bacterial pathogens.- Viability detection of foodborne bacterial pathogens in food environment by PMA-qPCR and by microscopy observation.- Electrochemical Detection of Nucleic Acids using 3D Graphene Screen Printed Electrodes.- Application of MinION long read sequencer for semi-targeted detection of foodborne pathogens.- Integrating MALDI-TOF mass spectrometry with machine learning techniques for rapid antimicrobial resistances screening of foodborne bacterial pathogens.- Assessment of viability of Listeria monocytogenes by flow cytometry. - Method to study the survival abilities of foodborne bacterial pathogens under food processing conditions.- Characterization of bacterial membrane fatty acid profiles for biofilm cells.- Comparing gene expression between planktonic and biofilm cells of foodborne bacterial pathogens through RT-qPC,- Characterization of Food-Borne Pathogens in Biofilms: A 4D Structural Dynamics Approach Using HCS-CLSM.- Zebrafish larvae microinjection and automated fluorescence microscopy for studying Klebsiella pneumoniae infection and the host immune response.- Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of hypervirulent Klebsiella pneumoniae in Dictyostelium discoideum.- A Comprehensive Guide to Quality Assessment and Data Submission for Genomic Surveillance of Enteric Pathogens.- Genomic pipeline for analysis of mutational events in bacteria.- Using GWAS and Machine Learning to identify and predict genetic variants associated with foodborne bacteria phenotypic traits.- Identifying putative biomarkers of foodborne pathogens using a metabolomic approach.- Illumina MiSeq 16S rRNA Gene Library Preparation for Poultry Processing Microbiome Analyses.- Bioinformatic pipeline for profiling foodborne bacterial ecology and resistome from short-read metagenomics.
Direct or DNA extraction-free amplification and quantification of foodborne pathogens.- The use of multiplex real time PCR for the simultaneous detection of foodborne bacterial pathogens.- Viability detection of foodborne bacterial pathogens in food environment by PMA-qPCR and by microscopy observation.- Electrochemical Detection of Nucleic Acids using 3D Graphene Screen Printed Electrodes.- Application of MinION long read sequencer for semi-targeted detection of foodborne pathogens.- Integrating MALDI-TOF mass spectrometry with machine learning techniques for rapid antimicrobial resistances screening of foodborne bacterial pathogens.- Assessment of viability of Listeria monocytogenes by flow cytometry. - Method to study the survival abilities of foodborne bacterial pathogens under food processing conditions.- Characterization of bacterial membrane fatty acid profiles for biofilm cells.- Comparing gene expression between planktonic and biofilm cells of foodborne bacterial pathogens through RT-qPC,- Characterization of Food-Borne Pathogens in Biofilms: A 4D Structural Dynamics Approach Using HCS-CLSM.- Zebrafish larvae microinjection and automated fluorescence microscopy for studying Klebsiella pneumoniae infection and the host immune response.- Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of hypervirulent Klebsiella pneumoniae in Dictyostelium discoideum.- A Comprehensive Guide to Quality Assessment and Data Submission for Genomic Surveillance of Enteric Pathogens.- Genomic pipeline for analysis of mutational events in bacteria.- Using GWAS and Machine Learning to identify and predict genetic variants associated with foodborne bacteria phenotypic traits.- Identifying putative biomarkers of foodborne pathogens using a metabolomic approach.- Illumina MiSeq 16S rRNA Gene Library Preparation for Poultry Processing Microbiome Analyses.- Bioinformatic pipeline for profiling foodborne bacterial ecology and resistome from short-read metagenomics.
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