This introductory text provides a contextual history and overview of the science and use of DNA typing methods. Logically organized, with clear, concise language, this manual ensures a fundamental understanding of forensic DNA analysis and a thorough background of the molecular techniques used to determine an individual's identity. The experiments cover DNA extraction, concentration, and assessment; DNA analysis using restriction fragment length polymorphisms; polymerase chain reaction and PCR-based typing tests; short tandem repeat analysis; and mitochondrial DNA analysis. There is also an…mehr
This introductory text provides a contextual history and overview of the science and use of DNA typing methods. Logically organized, with clear, concise language, this manual ensures a fundamental understanding of forensic DNA analysis and a thorough background of the molecular techniques used to determine an individual's identity. The experiments cover DNA extraction, concentration, and assessment; DNA analysis using restriction fragment length polymorphisms; polymerase chain reaction and PCR-based typing tests; short tandem repeat analysis; and mitochondrial DNA analysis. There is also an extensive glossary of DNA typing terminology and basic terms used in molecular biology.
An Overview of Forensic DNA Analysis, Types of Biological Samples, Exercise 1 DNA Extraction, Exercise 2 Concentration of Extracted DNA, Exercise 3 Microcon Concentration and Purification of Extracted DNA, Exercise 4 Assessing the Quality and Quantity of Isolated DNA, Exercise 5 DNA Analysis Using Restriction Fragment Length, Polymorphism (RFLP), Polymerase Chain Reaction (PCR), Exercise 6 Polymerase Chain Reaction (PCR) Based Tests: The AmpliType PM/DQA1 System, Exercise 7 Polymerase Chain Reaction (PCR) Based Tests: The D1S80 System, Experiment 8 Polymerase Chain Reaction (PCR) Based Tests: Short Tandem Repeat (STR) Analysis, Experiment 9 Polymerase Chain Reaction (PCR) Based Tests: Short Tandem Repeat (STR) Analysis to Determine Paternity (a Case Study), Experiment 10 Polymerase Chain Reaction (PCR) Based Tests: Y Chromosome Short Tandem Repeat (Y STR) Analysis (a Case Study), Exercise 11 Mitochondrial DNA (mtDNA) Analysis, Exercise 12 Assessment of Lumigraph and Autoradiograph Data, References, Appendices
An Overview of Forensic DNA Analysis, Types of Biological Samples, Exercise 1 DNA Extraction, Exercise 2 Concentration of Extracted DNA, Exercise 3 Microcon Concentration and Purification of Extracted DNA, Exercise 4 Assessing the Quality and Quantity of Isolated DNA, Exercise 5 DNA Analysis Using Restriction Fragment Length, Polymorphism (RFLP), Polymerase Chain Reaction (PCR), Exercise 6 Polymerase Chain Reaction (PCR) Based Tests: The AmpliType PM/DQA1 System, Exercise 7 Polymerase Chain Reaction (PCR) Based Tests: The D1S80 System, Experiment 8 Polymerase Chain Reaction (PCR) Based Tests: Short Tandem Repeat (STR) Analysis, Experiment 9 Polymerase Chain Reaction (PCR) Based Tests: Short Tandem Repeat (STR) Analysis to Determine Paternity (a Case Study), Experiment 10 Polymerase Chain Reaction (PCR) Based Tests: Y Chromosome Short Tandem Repeat (Y STR) Analysis (a Case Study), Exercise 11 Mitochondrial DNA (mtDNA) Analysis, Exercise 12 Assessment of Lumigraph and Autoradiograph Data, References, Appendices
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