This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods…mehr
This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Forensic DNA Analysis: An Overview of the Laboratory Process. Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods. Manual Silica Based DNA Extractions. Applied Biosystems(TM) PrepFiler(TM) Forensic DNA Extraction Kit (Manual and Semi Automated via AutoMate Express(TM)). Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation. DNA Extraction of Bone through Demineralization. Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ(TM) Methods. DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards. Yield Gel via Quantitative Gel Electrophoresis. Quantitative PCR of Alu Repeats using PowerUp(TM) SYBR® Green Master Mix. Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit. QIAGEN's Investigator® Quantiplex Pro Kit. DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C). Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System. Applied Biosystems' GlobalFiler(TM) PCR Amplification Kit. QIAGEN's Investigator® 24plex QS and GO! PCR Amplification. Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis. Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer. Likelihood Ratio Calculation using LRmix Studio. Mitochondrial DNA Analysis. An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints. Detection of Latent DNA using a DNA Binding Dye. Rapid DNA Profile Development with Applied Biosystems RapidHIT(TM) ID System. Next Generation Sequencing: ForenSeq(TM) DNA Signature Prep Kit with the Illumina MiSeq FGx.
Forensic DNA Analysis: An Overview of the Laboratory Process. Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods. Manual Silica Based DNA Extractions. Applied Biosystems(TM) PrepFiler(TM) Forensic DNA Extraction Kit (Manual and Semi Automated via AutoMate Express(TM)). Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation. DNA Extraction of Bone through Demineralization. Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ(TM) Methods. DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards. Yield Gel via Quantitative Gel Electrophoresis. Quantitative PCR of Alu Repeats using PowerUp(TM) SYBR® Green Master Mix. Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit. QIAGEN's Investigator® Quantiplex Pro Kit. DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C). Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System. Applied Biosystems' GlobalFiler(TM) PCR Amplification Kit. QIAGEN's Investigator® 24plex QS and GO! PCR Amplification. Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis. Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer. Likelihood Ratio Calculation using LRmix Studio. Mitochondrial DNA Analysis. An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints. Detection of Latent DNA using a DNA Binding Dye. Rapid DNA Profile Development with Applied Biosystems RapidHIT(TM) ID System. Next Generation Sequencing: ForenSeq(TM) DNA Signature Prep Kit with the Illumina MiSeq FGx.
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