From cell division to endoreduplication - Moskalenko, Oleksandr
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This work presents an early approach to large-scale transcriptional profiling of the soybean seed developmental transition from rapid cell division to endoreduplication. In the absence of soybean genome sequence a suppression-subtractive hybridization approach coupled with partial sequencing of the resulting differentially expressed transcripts and subsequent custom microarray production was used. The resulting subset of transcripts specific to the developmental process being studied was profiled using cDNA microarrays as a time course from seven to twenty two days after flowering.

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Produktbeschreibung
This work presents an early approach to large-scale
transcriptional profiling of the soybean seed
developmental transition from rapid cell division to
endoreduplication. In the absence of soybean genome
sequence a suppression-subtractive hybridization
approach coupled with partial sequencing of the
resulting differentially expressed transcripts
and subsequent custom microarray production was used.
The resulting subset of transcripts specific to the
developmental process being studied was profiled
using cDNA microarrays as a time course from seven to
twenty two days after flowering.
Autorenporträt
Oleksandr Moskalenko, Ph.D.: Studied biochemistry and molecular
biology at Purdue University. He received his Ph.D. from the
Department of Agronomy where he researched early soybean seed
development.