Topological relation between spatial objects is one of the important research area frequently used in GIS, robotics, artificial intelligence, image processing etc. But most often it has been seen that spatial objects show discontinuities in boundary and exterior which give rise to the study of fuzzy regions with holes. In this book, we have studied in detail various kinds of fuzzy boundary. Then, we have provided models for fuzzy regions with holes and derive their properties and topological relations. Finally, we provide a methodolgy to show application of developed framework to real life situations.