The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. The book also discusses approaches to grant funding and SiRNA technology. This edition features new techniques in every chapter, as well as several new chapters.
The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. The book also discusses approaches to grant funding and SiRNA technology. This edition features new techniques in every chapter, as well as several new chapters.
William Wu, Ph.D., has extensive experience in both academic and industrial settings. He held various positions, including Research Assistant Professor at the University of Michigan Medical School, Ann Arbor, Michigan, USA, and Professor at the Hunan Nonnal University, Hunan, the People's Republic of China, Senior Scientist, Team-Leader and Chief Technology Officer (CTO) in biomedical and pharmaceutical companies. Helen H. Zhang, MS., is Senior Research Associate at the University of Michigan Medical School, Ann Arbor, Michigan, USA. In 1996, she received her MS degree in Molecular and Cellular Biology at the Eastern Michigan University, Ypsilanti, Michigan, USA. Michael J. Welsh, Ph.D., is Professor of Cell Biology in the Department of Cell and Developmental Biology at the University of Michigan Medical School, Ann Arbor, Michigan, USA. He is also a Professor of Toxicology in the School of Public Health at the University of Michigan, Ann Arbor. Peter B. Kanfman, Ph.D., is Professor of Biology in the Plant Cellular and Molecular Biology Program in the Department of Biology and a member of the faculty of the Bioengineering Program at the University of Michigan.
Inhaltsangabe
Strategies for Novel Research Projects and Research Grant Funding. Rapid Isolation of Specific cDNA or Gene by PCR. Construction and Screening of Subtracted and Complete Expression cDNA Libraries. Subcloning of Genes or DNA Fragments. Non-isotopic and Isotopic DNA or RNA Sequencing. Information Superhighway and Computer Databases of Nucleic Acids & Proteins. Characterization of DNA or Genes by Southern Blot Hybridization. Gene Over-expression by Sense RNA in Mammalian System. Gene Under-expression by Antisense DNA and RNA Strategies. Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR. Analysis of Gene Expression at the Proteomic Level via Western Blotting. Analysis of Protein Expression Using Immuno-fluorescence Localization. Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH). Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH). In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR. Detection of Low Abundance mRNA. Isolation and Characterization of Genes from Genomic DNA Libraries. Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression. New Strategies for Gene Knockout. Large-scale Expression and Purification of Recombinant Proteins. Quantitative Analysis of Functional Genome by Real-Time RT -PCR. High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips). Construction and Screening of Human Antibody Libraries Using Phage Display Technology. Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology. New Techniques for Identification of Protein Binding and Proteomic Interactions.
Strategies for Novel Research Projects and Research Grant Funding. Rapid Isolation of Specific cDNA or Gene by PCR. Construction and Screening of Subtracted and Complete Expression cDNA Libraries. Subcloning of Genes or DNA Fragments. Non-isotopic and Isotopic DNA or RNA Sequencing. Information Superhighway and Computer Databases of Nucleic Acids & Proteins. Characterization of DNA or Genes by Southern Blot Hybridization. Gene Over-expression by Sense RNA in Mammalian System. Gene Under-expression by Antisense DNA and RNA Strategies. Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR. Analysis of Gene Expression at the Proteomic Level via Western Blotting. Analysis of Protein Expression Using Immuno-fluorescence Localization. Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH). Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH). In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR. Detection of Low Abundance mRNA. Isolation and Characterization of Genes from Genomic DNA Libraries. Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression. New Strategies for Gene Knockout. Large-scale Expression and Purification of Recombinant Proteins. Quantitative Analysis of Functional Genome by Real-Time RT -PCR. High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips). Construction and Screening of Human Antibody Libraries Using Phage Display Technology. Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology. New Techniques for Identification of Protein Binding and Proteomic Interactions.
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