Gene Essentiality

Methods and Protocols
Herausgegeben:Lu, Long Jason

• Broschiertes Buch

Produktbeschreibung

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

Produktdetails

• Methods in Molecular Biology 1279
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-4862-8
• Softcover reprint of the original 1st ed. 2015
• Seitenzahl: 260
• Erscheinungstermin: 5. Oktober 2016
• Englisch
• Abmessung: 254mm x 178mm x 14mm
• Gewicht: 555g
• ISBN-13: 9781493948628
• ISBN-10: 1493948628
• Artikelnr.: 46000202

Inhaltsangabe

Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni.- Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation.- Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq).- Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12.- Identification of Genes Essential for Leptospirosis.- Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling.- Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis.- Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans.- An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes.- A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Essential Genes.- A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study.- Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches.- Three Computational Tools for Predicting Bacterial Essential Genes.- Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes.- A Novel Essential Domain Perspective for Exploring Gene Essentiality.