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Trichoderma is a genus of fungi in the family Hypocreaceae found in all types of soils and is the most common culturable fungus. They occur worldwide and can be easily isolated from soil, plant organic matter, decaying wood etc. It is a well-known aggressive biocontrol agent and has a higher potential for reproduction and colonisation. In this study the genetic diversity among fourteen Trichoderma isolates (TBT- 01 to TBT- 14) was evaluated using ten SSR markers. Rhizospheric soil samples from various locations of Uttar Pradesh were collected and subjected to serial dilution and plating on a Trichoderma Selective Media. The isolates' colonies were pure cultured on Potato Dextrose Agar for DNA isolation and molecular studies. Ten SSR primers (SSR-01, SSR- 06, SSR- 08, SSR- 10, SSR- 11, SSR-13, SSR- 14, SSR- 15, SSR- 18, and SSR- 20) were used for the PCR amplification of the DNA isolated.