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Study on correlation and path coefficient analysis for cane yield and yield related traits in ten accession of sugarcane (Saccharum officinarum L.) was conducted Analysis of variance indicated highly significant differences among the accessions for all the traits. The study of path analysis for yield related traits depicted that plant height, cane thickness, leaf area, internodal distance and brix value had the direct negative effects on yield. Study carried out to investigate the response of different sugarcane varieties to Callogenesis in order to analyze variation for sugarcane mosaic virus resistance. Total 26 parent plants and 64 somaclones of HSF-240 were selected for screening of virus through Das ELISA test. Four parent plants out of 26 showed negative and Ten somaclones showed positive reaction to the disease and were declared as susceptible.Somaclones produced were also analyzed by microsattelite molecular markers in order to analyze the vriation between parents and somaclones produced at molecular level.It may be concluded from the results that SSRs can be used for identification of somaclonal variation and the relationship between parents and somaclones.