Genetic transformation of rice with gshf for abiotic stress tolerance - Srivashtav, Vishal;Jha, Sanjay;Parekh, Vipul
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Rice (Oryza sativa L.) is a staple food crop which greatly affected by different environmental stresses such as drought, salt and low temperature. Glutathione gshF, the tripeptide is a multifunctional metabolite in plants. A novel bifunctional enzyme from the Listeria monocytogenes), has been used to develop transgenic rice plants under 35s CaMV promoter in the background of indica rice cultivar NAUR-1 through Agrobacterium-mediated transformation. Total of 200 scutellum-derived embryogenic calli was used for transformation, screened by PCR namely gshF-5, gshF-12 and gshF-19, were obtained…mehr

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Produktbeschreibung
Rice (Oryza sativa L.) is a staple food crop which greatly affected by different environmental stresses such as drought, salt and low temperature. Glutathione gshF, the tripeptide is a multifunctional metabolite in plants. A novel bifunctional enzyme from the Listeria monocytogenes), has been used to develop transgenic rice plants under 35s CaMV promoter in the background of indica rice cultivar NAUR-1 through Agrobacterium-mediated transformation. Total of 200 scutellum-derived embryogenic calli was used for transformation, screened by PCR namely gshF-5, gshF-12 and gshF-19, were obtained with 1.5% transformation frequency. The gshF gene was stably inherited and expressed in T2 plants, as indicated by the results of PCR, ELISA, RT-PCR and qRT-PCR analyses. Average expression of gshF was 3.03 µg/mg of total soluble protein in leaf extracts of transgenic plants. qRT-PCR analysis of gshF showed 5.83 and 6.07 higher fold in gshF-5-7 and gshF-5-8 at 200 mM NaCl treatment as compare to control. In transformed plant, glutathione content was increased more than 14 fold as compared to untransformed plant at 200 mM NaCl treatment.
Autorenporträt
Vishal Srivashtav is Biotechnologist holding a post graduate degree in Plant Biotechnology from Navsari Agricultural university, Gujarat, India. He is currently pursuing Ph.D. as Research scholar from navsari Agricultural university and working on heavy metal tolerance in castor.