The book has been arranged in five parts.Part one,Introduction, describes short overview of Rice plant, its importance and importance of Genetic transformation for improving crop variety.Part two, Review of literature covers the views of different authors and about the researches has been done on genetic transformation in different crops.Part third,Materials and methods, contains the standardized protocol of agrobacterium mediated transformation in rice with the materials used in this experiments.Part forth,Results and Discussion,describes the effect of cystein and dithiothretol on agrobacterium mediated transformation in rice. Part fifth, Conclusion, deals with importance of these two compounds for increasing the transformation efficiency.It will be useful for the researchers and students who works on Genetic tranformation by virtue of its authoriative coverage.