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Aqueous solutions of glycols have attracted much attention. These solutions have been used in biochemistry, polymer chemistry and biochemical engineering to separate and purify biological products, biomaterials, proteins, and enzymes from the complex mixtures. The aim of this work is: provide information on the molecular interactions between water, glycols in binary mixtures at different temperatures, to investigate the volumetric and viscometric behaviours of these binary aqueous systems, and correlate the density and viscosity data to the thermodynamics of mixing in terms of Transition State Theory, and the thermodynamic functions of activation for viscous flow of these solutions.